Review



ly3000328  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    MedChemExpress ly3000328
    The effects of CTSS inhibitor <t>LY3000328</t> on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.
    Ly3000328, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ly3000328/product/MedChemExpress
    Average 94 stars, based on 19 article reviews
    ly3000328 - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Cathepsin S contributes to influenza-induced lung injury by driving inflammation, promoting apoptosis, and disrupting epithelial barrier integrity"

    Article Title: Cathepsin S contributes to influenza-induced lung injury by driving inflammation, promoting apoptosis, and disrupting epithelial barrier integrity

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.01128-25

    The effects of CTSS inhibitor LY3000328 on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.
    Figure Legend Snippet: The effects of CTSS inhibitor LY3000328 on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.

    Techniques Used: Infection, Injection, Virus, Control, Histopathology, Gene Expression, Activity Assay, Staining, Expressing, Quantitative RT-PCR



    Similar Products

    ly3000328  (MedChemExpress)
    94
    MedChemExpress ly3000328
    The effects of CTSS inhibitor <t>LY3000328</t> on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.
    Ly3000328, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ly3000328/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    ly3000328 - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    ly 3000328  (MedChemExpress)
    94
    MedChemExpress ly 3000328
    The effects of CTSS inhibitor <t>LY3000328</t> on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.
    Ly 3000328, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ly 3000328/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    ly 3000328 - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    hy 15533 fitc dextran mw 4000 medchemexpress  (MedChemExpress)
    94
    MedChemExpress hy 15533 fitc dextran mw 4000 medchemexpress
    The effects of CTSS inhibitor <t>LY3000328</t> on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.
    Hy 15533 Fitc Dextran Mw 4000 Medchemexpress, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hy 15533 fitc dextran mw 4000 medchemexpress/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    hy 15533 fitc dextran mw 4000 medchemexpress - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    The effects of CTSS inhibitor LY3000328 on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.

    Journal: Microbiology Spectrum

    Article Title: Cathepsin S contributes to influenza-induced lung injury by driving inflammation, promoting apoptosis, and disrupting epithelial barrier integrity

    doi: 10.1128/spectrum.01128-25

    Figure Lengend Snippet: The effects of CTSS inhibitor LY3000328 on influenza infection in mice. ( A ) Schematic diagram of the experimental design. C57BL/6J mice ( n = 5 per group) received intraperitoneal injection of 30 mg/kg LY3000328 or a placebo vehicle twice daily for 5 days. Four hours after the initial dose, mice were intranasally inoculated with 100 PFU of PR8 virus. A mock control group received the vehicle orally twice daily and PBS intranasally. Body weight was monitored daily. Mouse lungs were collected at 5 dpi for the analyses of viral titers, histopathology, and gene expression. ( B ) CTSS activity in lung homogenate among different groups. ( C ) Viral titers in lung homogenates determined using the TCID 50 assay in MDCK cells. ( D ) Body weight change of mice across the experimental period. ( E ) Survival curve of mice across the experimental period. ( F ) Histopathological examination of mouse lungs performed by H&E staining. ( G ) Analysis of mRNA expression levels of cytokines in lung tissues by RT-qPCR. Data are represented as mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant.

    Article Snippet: For CTSS inhibition, mice received intraperitoneal injections of 30 mg/kg LY3000328 (MedChem Express, USA, HY-15533) or vehicle twice daily for 5 days.

    Techniques: Infection, Injection, Virus, Control, Histopathology, Gene Expression, Activity Assay, Staining, Expressing, Quantitative RT-PCR